Journal: European Journal of Dentistry

Article Title: Gingival-Derived Mesenchymal Stem Cell from Rabbit ( Oryctolagus cuniculus ): Isolation, Culture, and Characterization

doi: 10.1055/s-0040-1719213

Figure Lengend Snippet: The morphology of GDMSCs at the fourth passage shows a spindle-like formation and fibroblast-like cells attached to the base of the plate culture. Examination using the immunohistochemical method with green immunofluorescence staining was observed using inverted fluorescence microscopy with ×100 magnification. ( A ) CD44 positive expression; ( B ) CD73 positive expression; ( C ) CD90 positive expression; ( D ) CD105 positive expression; and ( E ) CD200 positive expression. CD, cluster of differentiation; GDMSCs, gingival-derived mesenchymal stem cells.

Article Snippet: Polyclonal antibodies CD34, CD44, CD45, CD73, CD90, and CD105 labeled FITC (Bioss Antibodies Inc.) are added.

Techniques: Immunohistochemical staining, Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing, Derivative Assay

Journal: European Journal of Dentistry

Article Title: Gingival-Derived Mesenchymal Stem Cell from Rabbit ( Oryctolagus cuniculus ): Isolation, Culture, and Characterization

doi: 10.1055/s-0040-1719213

Figure Lengend Snippet: Characterization of the fourth passage of the GDMSCs with flow cytometry examination. ( A ) GDMSCs expressed CD105 but not CD45; ( B ) GDMSCs also expressed MSC markers, namely, CD44, CD73, and CD90 expressions. CD, cluster of differentiation; GDMSCs, gingival-derived mesenchymal stem cells.

Article Snippet: Polyclonal antibodies CD34, CD44, CD45, CD73, CD90, and CD105 labeled FITC (Bioss Antibodies Inc.) are added.

Techniques: Flow Cytometry, Derivative Assay

Journal: Annals of Translational Medicine

Article Title: Characterization of wild-type and STAT3 signaling-suppressed mesenchymal stem cells obtained from hemovac blood concentrates

doi: 10.21037/atm-21-791

Figure Lengend Snippet: Expression of surface markers in OA-MSCs and iSTAT3-MSCs. (A) OA-MSCs; (B) iSTAT3-MSCs. The expression of the typical MSC markers—CD73, CD90, and CD105—was high (>90%) in both OA-MSCs and iSTAT3-MSCs. The negative markers—CD34, CD45, and HLA-DR—were barely expressed (<4%) in OA-MSCs and iSTAT3-MSCs. Blue: Isotype control expression. Red: Surface marker expression. OA-MSCs, mesenchymal stem cells in patients with osteoarthritis; STAT3, signal transducer and activator of transcription 3.

Article Snippet: The antibodies used in this study were: anti-CD73 (ecto-5'-nucleotidase; Phycoerythrin (PE)-labeled mouse anti-human CD73, clone AD2, 550257, BD Biosciences, Heidelberg, Germany), anti-CD90 (thy-1; allophycocyanin (APC)-labeled mouse anti-human CD90, clone 5E10, 17-0909, eBioscience, Waltham, MA, USA), anti-CD105 (Endoglin; PE-labeled mouse anti-human CD105, clone 266, 560839, BD Biosciences), anti-CD34 (hematopoietic progenitor cell antigen CD34; Fluorescein isothiocyanate (FITC)-labeled mouse anti-human CD34, clone 581, 560942, BD Biosciences), anti-CD45 (leukocyte common antigen; PE-labeled mouse anti-human CD45, clone HI30, 555483, BD Biosciences), HLA-DR (MHC class II antigen; FITC-labeled mouse anti-human HLA-DR, clone G46-6, 555811, BD Biosciences).

Techniques: Expressing, Control, Marker

Journal: Scientific Reports

Article Title: Therapeutic effect of mesenchymal stem cells derived from human umbilical cord in rabbit temporomandibular joint model of osteoarthritis

doi: 10.1038/s41598-019-50435-2

Figure Lengend Snippet: Surface marker analysis by flow cytometry. hUCM-MSCs were negative for CD34 (0.4%) and CD45 (0.3%), and positive for CD90 (98.8%) and CD105 (99.8%).

Article Snippet: Antibodies against CD34-PE, CD45-PE, CD90-PE and CD105-PE (PE-labeled; BD Biosciences, Franklin Lake, NJ, USA) were used.

Techniques: Marker, Flow Cytometry

Journal: Frontiers in Veterinary Science

Article Title: Evaluation of stability and safety of equine mesenchymal stem cells derived from amniotic fluid for clinical application

doi: 10.3389/fvets.2024.1330009

Figure Lengend Snippet: Stem cell marker variation. Relative gene expression level was normalized by expression level of target gene of skin fibroblast (A) . The graph and error bar represent mean of ΔCT value ± SEM. Significant difference represented * ( p = 0.0016) and ** ( p < 0.001). Cell surface marker analysis of AF-MSC at passage 5 to 7 by FACS (B) . The AF-MSC were positive for CD29, CD44, CD90, and CD105, and negative for CD14, CD34, CD38, CD45, and MHC class II. Expression of IgG was used for negative control.

Article Snippet: Cells were fixed with 4% paraformaldehyde (Thermo Fisher Scientific, MA, USA), and stained with antibodies against CD29 (phycoerythrin [PE]-labeled anti-human CD29 antibody; BioLegend, San Diego, CA, USA), CD44 (PE-labeled anti-mouse/human CD44 antibody; BioLegend), CD90 (PE-labeled mouse anti-rat CD90/mouse CD90.1; BD Biosciences), CD105 (fluorescein isothiocyanate [FITC]-labeled mouse anti-human CD105; Bio-Rad, Hercules, CA, USA), CD14 (porcine/equine CD14 antibody, R&D Systems, Minneapolis, MM, USA), CD34 (FITC-labeled mouse anti-human CD34; BD Pharmigen, Franklin Lakes, NJ, USA), CD45 (FITC-labeled mouse anti-human CD45; Southern Biotech, Birmingham, AL, USA), and major histocompatibility class II (FITC-labeled MHC class II antibody, clone CVS20; LSBio, Seattle, WA, USA).

Techniques: Marker, Gene Expression, Expressing, Negative Control

Journal: Cancer Research

Article Title: Adipose Tissue–Derived Human Mesenchymal Stem Cells Mediated Prodrug Cancer Gene Therapy

doi: 10.1158/0008-5472.can-06-4024

Figure Lengend Snippet: Figure 2. Retrovirus transduction does not affect cell surface marker expression and differentiation properties and directed migration toward colon carcinoma cells HT-29 in transgene-expressing CD-AT- MSC derived from cultured AT-MSC. A, AT-MSC and CD-AT-MSC were labeled with fluorophore-conjugated antibodies and analyzed by flow cytometry (red histograms). Mouse isotype antibodies IgG1 and IgG2a served as respective controls (white histograms). Both cell types were positive for CD29, CD44, CD90, and CD105. B, AT-MSC and CD-AT-MSC were cultured in medium supplemented with components for adipogenic differentiation for 28 d. Cells were stained with Oil Red-O. Red-stained oil droplets visible in AT-MSC and CD-AT-MSC were indicative of adipogenic differentiation. Both cell types were found to be capable of adipogenic differentiation. Magnification, 80. C, AT-MSC and CD-AT-MSC were cultured in medium supplemented with components for osteogenic differentiation. Cells were stained with Alizarin red S 28 d later. Red-stained calcium deposits were detected in differentiated cultured AT-MSC and CD-AT-MSC. Both transgene- expressing CD-AT-MSC and AT-MSC were capable of osteogenic differentiation. Magnification, 160. D, migratory ability of MSC was determined using a cell invasion assay kit. AT-MSC and CD-AT- MSC were loaded onto ECMatrix-coated inserts and incubated with HT-29 cells in serum-free medium. Cell migration was evaluated after staining under microscope 48 h later by taking photographs and calculating number of migrated cells. Fibroblasts were used as negative control. Columns, mean; bars, SE. There was no significant difference in the migration capabilities of transgene-expressing CD-AT-MSC compared with AT-MSC. However, tumor cells significantly stimulate the migration of AT-MSC cells compared with fibroblasts. Magnification, 40. *, P < 0.05, Mann-Whitney U test.

Article Snippet: MSC and HT-29 were labeled with the following antihuman antibodies conjugated to FITC: CD14-FITC, CD34-FITC, CD44-FITC, CD45-FITC, and CD105-FITC (purchased from Santa Cruz www.aacrjournals.org 6305 Cancer Res 2007; 67: (13).

Techniques: Transduction, Marker, Expressing, Migration, Derivative Assay, Cell Culture, Labeling, Flow Cytometry, Staining, Invasion Assay, Incubation, Microscopy, Negative Control, MANN-WHITNEY